Immunohistochemical staining for the p53 protein and proliferating cell nuclear antigen in familial clustering of gastric cancer

✍ Scribed by Okusa, Yasushi; Ichikura, Takashi; Tamakuma, Shoetsu

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 504 KB
• Volume: 62
• Category: Article
• ISSN: 0022-4790
• DOI: 10.1002/(sici)1096-9098(199608)62:4<253::aid-jso5>3.0.co;2-5

✦ Synopsis

Purpose of this study was to assess the role of p53 gene and tumor proliferating activity in familial clustering of gastric cancer.

Materials and Methods: Among 344 patients who underwent resections for gastric cancer, 10 patients had two or more gastric cancer-affected, first-degree relatives. We classified them as the group of gastric cancer with family history (FGC). Eighty-seven patients with gastric cancer who had no relatives with any malignant neoplasm were classified as the sporadic group. The paraffin-embedded specimens were stained immunohistochemically using monoclonal antibodies against the p53 product and proliferating cell nuclear antigen (PCNA).

Results: There was no significant difference in any clinicopathologic factor and the PCNA labeling index between the two groups. Staining for the p53 product was positive in 80% of the FGC group and in 38% of the sporadic group ( P < 0.05).

Conclusion:

Our study suggests that overexpression of p53 protein is one of the familial factors that correlates with carcinogenesis in the stomach.