NovelhMSH2,hMSH6 andhMLH1 gene mutations and microsatellite instability in sporadic colorectal cancer
β Scribed by P. Chaksangchaichot; P. Punyarit; S. Petmitr
- Publisher
- Springer-Verlag
- Year
- 2006
- Tongue
- English
- Weight
- 322 KB
- Volume
- 133
- Category
- Article
- ISSN
- 1432-1335
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The reproducibility of microsatellite instability from different regions of the same sporadic colon cancer has not been addressed. We therefore microdissected and extracted DNA from three to nine separate regions of 13 highly unstable sporadic colon cancers. Each region was then evaluated by polymer
The microsatellite instability that is a feature of tumors in patients with hereditary nonpolyposis colorectal cancer (HNPCC) is a consequence of defective DNA mismatch repair. Mutations in the DNA mismatch repair genes MSH2 and MLH1 may account for up to 90% of HNPCC kindreds. Microsatellite instab
We have screened 17 Southern Sweden individuals/families with suspected hereditary non-polyposis colorectal cancer (HNPCC) for mutations in the DNA-mismatch repair genes hMLH1, hMSH2 and hMSH6 using denaturing gradient gel electrophoresis, protein truncation test and direct DNA sequencing. The famil