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Normal-phase Liquid Chromatography Class Separation and Species Determination of Phospholipids Utilizing Electrospray Mass Spectrometry/Tandem Mass Spectrometry

✍ Scribed by Anders Å Karlsson; Peter Michelsen; Åsmund Larsen; Göran Odham

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
408 KB
Volume
10
Category
Article
ISSN
0951-4198

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✦ Synopsis

Membrane phospholipids have been separated into classes by normal-phase chromatography using electrospray (ES) mass spectrometry or with tandem mass spectrometry (MS/MS) for species determination. Biological samples, taken from patients seeking a remedy for gastric catarrh or gastric ulcer were examined. It was found, by altering the cone voltage and changing between positive-and negative-ion modes in ES, that in most cases it was possible to obtain structural information for the molecular species and fatty acids present. MSfMS was employed to achieve accurate species determination.

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