๐”– Bobbio Scriptorium
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Nonaqueous oxidation of phosphites to phosphates in nucleotide synthesis

โœ Scribed by Kelvin K. Ogilvie; Mona J. Nemer


Book ID
104241706
Publisher
Elsevier Science
Year
1981
Tongue
French
Weight
263 KB
Volume
22
Category
Article
ISSN
0040-4039

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โœฆ Synopsis


m-Chloroperbenzoic acid in methylene chloride represents a rapid non-aqueous method for the conversion of phosphites to phosphates during oligonucleotide synthesis both in solution and on a solid support.


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Summay : Bis (trimethylsilyl) peroxide, t-butyl hydroperoxide, cumene hydroperoxide, and N-methylmorpholine N-oxide serve as excellent agents for anhydrous oxidation of nucleoside phosphites to the phosphates. The reaction using bis (trimethylsilyl) peroxide is conspicuously accelerated by added tr

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Phosphates linked to anomeric carbon atoms have been reported to be present at the cell surface of such microbes as Staphylococcus lactis2, Saccharomyces cerevisiae3, Micrococcus lysodeikticufl , and Streptococcus pneumoniae' , and to be a part of the structure of glycan chains of glycoproteins' . T