NMR-Based Metabolomics: Methods and Protocols

✍ Scribed by G. A. Nagana Gowda, Daniel Raftery

• Publisher: Springer New York;Humana
• Year: 2019
• Tongue: English
• Leaves: 476
• Series: Methods in Molecular Biology 2037
• Edition: 1st ed. 2019
• Category: Library

✦ Synopsis

This book provides broad coverage of nuclear magnetic resonance (NMR) spectroscopy-based methods and applications for the analysis of metabolites in a wide range of biological samples, from biofluids, cells, animal models, human, to plants and foods. The applications range from mechanistic understanding, biomarker discovery, environmental studies, and drug discovery to nutrition, while NMR methods include global, targeted, and isotope tracer-based techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, NMR-Based Metabolomics: Methods and Protocols serves as a wealth of information for beginners as well as advanced practitioners and also as stepping stones for further advances in the field of metabolomics.

✦ Table of Contents

Front Matter ....Pages i-xiv
Front Matter ....Pages 1-1
Overview of NMR Spectroscopy-Based Metabolomics: Opportunities and Challenges (G. A. Nagana Gowda, Daniel Raftery)....Pages 3-14
Front Matter ....Pages 15-15
Analysis of Plasma, Serum, and Whole Blood Metabolites Using 1H NMR Spectroscopy (G. A. Nagana Gowda, Daniel Raftery)....Pages 17-34
Lipid Profiling Using 1H NMR Spectroscopy (Miriam Gil, Sara Samino, Rubén Barrilero, Xavier Correig)....Pages 35-47
High-Resolution Magic Angle Spinning (HRMAS) NMR Methods in Metabolomics (Marlon Tilgner, Tim S. Vater, Piet Habbel, Leo L. Cheng)....Pages 49-67
Practical Guidelines for 13C-Based NMR Metabolomics (Arthur S. Edison, Adrien Le Guennec, Frank Delaglio, Ēriks Kupče)....Pages 69-95
Analysis of Coenzymes and Antioxidants in Tissue and Blood Using 1D 1H NMR Spectroscopy (G. A. Nagana Gowda, Daniel Raftery)....Pages 97-110
Front Matter ....Pages 111-111
UHPLC-QTOF-MS/MS-SPE-NMR: A Solution to the Metabolomics Grand Challenge of Higher-Throughput, Confident Metabolite Identifications (Anil Bhatia, Saurav J. Sarma, Zhentian Lei, Lloyd W. Sumner)....Pages 113-133
Fast NMR Methods for Identification of Resonances and Metabolic Pathways (Abhinav Dubey, Shivanand M. Pudakalakatti, Hanudatta S. Atreya)....Pages 135-147
Front Matter ....Pages 149-149
Stable Isotope-Resolved Metabolomics by NMR (Penghui Lin, Andrew N. Lane, Teresa W.-M. Fan)....Pages 151-168
Tracking Metabolic Rewiring of Cancer Metabolism in Humans Using Isotope-Resolved NMR (Kumar Pichumani)....Pages 169-186
Front Matter ....Pages 187-187
NMR-Based Metabolomics in Cardiac Research (Julian L. Griffin, Cecilia Castro)....Pages 189-194
Breast Cancer Metabolomics Using NMR (Uma Sharma, Naranamangalam R. Jagannathan)....Pages 195-213
NMR-Based Urinary Metabolomics Applications (Tedros Bezabeh, Ana Capati, Omkar B. Ijare)....Pages 215-229
NMR-Based Metabolomics in Gallbladder Cancer Research (Renuka Ranjan, Neeraj Sinha)....Pages 231-241
Biomarker Discovery Using NMR-Based Metabolomics of Tissue (Maria T. Grinde, Guro F. Giskeødegård, Trygve Andreassen, May-Britt Tessem, Tone F. Bathen, Siver A. Moestue)....Pages 243-262
Front Matter ....Pages 263-263
NMR Metabolomics Protocols for Drug Discovery (Fatema Bhinderwala, Robert Powers)....Pages 265-311
Front Matter ....Pages 313-313
Practical Aspects of the Analysis of Low- and High-Field NMR Data from Environmental Samples (Jun Kikuchi)....Pages 315-331
Front Matter ....Pages 333-333
NMR-Based Metabolomics of Food (Ulrik K. Sundekilde, Nina Eggers, Hanne Christine Bertram)....Pages 335-344
Plant Metabolomics Using NMR Spectroscopy (Denise Medeiros Selegato, Alan Cesar Pilon, Fausto Carnevale Neto)....Pages 345-362
Front Matter ....Pages 363-363
Fast Quantitative 2D NMR for Untargeted and Targeted Metabolomics (Estelle Martineau, Patrick Giraudeau)....Pages 365-383
Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization (Mathilde H. Lerche, Magnus Karlsson, Jan H. Ardenkjær-Larsen, Pernille R. Jensen)....Pages 385-393
Metabolic Profiling Using In Vivo High Field Flow NMR (Maryam Tabatabaei Anaraki, Daniel Lane, Monica Bastawrous, Amy Jenne, André J. Simpson)....Pages 395-409
Front Matter ....Pages 411-411
Tools for Enhanced NMR-Based Metabolomics Analysis (John L. Markley, Hesam Dashti, Jonathan R. Wedell, William M. Westler, Hamid R. Eghbalnia)....Pages 413-427
Automated Tools for the Analysis of 1D-NMR and 2D-NMR Spectra (Matthias Lipfert, Manoj Kumar Rout, Mark Berjanskii, David S. Wishart)....Pages 429-449
Front Matter ....Pages 451-451
Processing and Analysis of Untargeted Multicohort NMR Data (Timothy M. D. Ebbels, Ibrahim Karaman, Gonçalo Graça)....Pages 453-470
Advanced Statistical Methods for NMR-Based Metabolomics (Dabao Zhang, Min Zhang)....Pages 471-482
Back Matter ....Pages 483-489

✦ Subjects

Life Sciences; Metabolomics; Spectroscopy/Spectrometry