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Protein NMR: Methods and Protocols

✍ Scribed by Ranajeet Ghose (eds.)


Publisher
Humana Press
Year
2018
Tongue
English
Leaves
448
Series
Methods in Molecular Biology 1688
Edition
1
Category
Library

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✦ Synopsis


This volume covers state-of-the-art applications of solid-state and solution nuclear magnetic resonance( NMR) spectroscopy to study protein structure, dynamics and interactions. Chapters detail various aspects of data acquisition and processing, determination of the structure, multi-timescale dynamics of entities ranging from individual proteins to large macromolecular complexes to intact viral assemblies. The final two chapters will highlight the promise of NMR beyond field strengths of 1 GHz to study the structure, dynamics and interactions of a larger class of proteins and protein complexes of extraordinary biological interest. Written in the highly successful Methods in Molecular Biology series format, chapters provide detailed laboratory protocols and troubleshooting tips that would be of great practical help to NMR spectroscopists with different levels of expertise.

Authoritative and cutting-edge, Protein NMR: Methods and Protocol aims to ensure successful results in the further study of this vital field.

✦ Table of Contents


Front Matter ....Pages i-xiii
NMR of Macromolecular Assemblies and Machines at 1 GHz and Beyond: New Transformative Opportunities for Molecular Structural Biology (Caitlin M. Quinn, Mingzhang Wang, Tatyana Polenova)....Pages 1-35
Experimental Aspects of Polarization Optimized Experiments (POE) for Magic Angle Spinning Solid-State NMR of Microcrystalline and Membrane-Bound Proteins (T. Gopinath, Gianluigi Veglia)....Pages 37-53
Afterglow Solid-State NMR Spectroscopy (Gili Abramov, Nathaniel J. Traaseth)....Pages 55-66
Filamentous Bacteriophage Viruses: Preparation, Magic-Angle Spinning Solid-State NMR Experiments, and Structure Determination (Omry Morag, Nikolaos G. Sgourakis, Gili Abramov, Amir Goldbourt)....Pages 67-97
Spherical Nanoparticle Supported Lipid Bilayers: A Tool for Modeling Protein Interactions with Curved Membranes (Erin R. Tyndall, Fang Tian)....Pages 99-109
Rapid Prediction of Multi-dimensional NMR Data Sets Using FANDAS (Siddarth Narasimhan, Deni Mance, Cecilia Pinto, Markus Weingarth, Alexandre M. J. J. Bonvin, Marc Baldus)....Pages 111-132
Strategies for Efficient Sample Preparation for Dynamic Nuclear Polarization Solid-State NMR of Biological Macromolecules (Boris Itin, Ivan V. Sergeyev)....Pages 133-154
In-Vitro Dissolution Dynamic Nuclear Polarization for Sensitivity Enhancement of NMR with Biological Molecules (Yaewon Kim, Yunyi Wang, Hsueh-Ying Chen, Christian Hilty)....Pages 155-168
Determination of Protein ps-ns Motions by High-Resolution Relaxometry (Samuel F. Cousin, Pavel Kadeřávek, Nicolas Bolik-Coulon, Fabien Ferrage)....Pages 169-203
Characterizing Protein Dynamics with NMR R1ρ Relaxation Experiments (Francesca Massi, Jeffrey W. Peng)....Pages 205-221
CPMG Experiments for Protein Minor Conformer Structure Determination (Anusha B. Gopalan, D. Flemming Hansen, Pramodh Vallurupalli)....Pages 223-242
Probing the Atomic Structure of Transient Protein Contacts by Paramagnetic Relaxation Enhancement Solution NMR (Vincenzo Venditti, Nicolas L. Fawzi)....Pages 243-255
From Raw Data to Protein Backbone Chemical Shifts Using NMRFx Processing and NMRViewJ Analysis (Bruce A. Johnson)....Pages 257-310
Protein Structure Elucidation from NMR Data with the Program Xplor-NIH (Guillermo A. Bermejo, Charles D. Schwieters)....Pages 311-340
Practical Nonuniform Sampling and Non-Fourier Spectral Reconstruction for Multidimensional NMR (Mark W. Maciejewski, Adam D. Schuyler, Jeffrey C. Hoch)....Pages 341-352
Covariance NMR Processing and Analysis for Protein Assignment (Bradley J. Harden, Dominique P. Frueh)....Pages 353-373
Structures of Dynamic Protein Complexes: Hybrid Techniques to Study MAP Kinase Complexes and the ESCRT System (Wolfgang Peti, Rebecca Page, Evzen Boura, Bartosz Różycki)....Pages 375-389
Implementation of the NMR CHEmical Shift Covariance Analysis (CHESCA): A Chemical Biologist’s Approach to Allostery (Stephen Boulton, Rajeevan Selvaratnam, Rashik Ahmed, Giuseppe Melacini)....Pages 391-405
High-Efficiency Expression of Yeast-Derived G-Protein Coupled Receptors and 19F Labeling for Dynamical Studies (Libin Ye, Alexander P. Orazietti, Aditya Pandey, R. Scott Prosser)....Pages 407-421
Quantitative Determination of Interacting Protein Surfaces in Prokaryotes and Eukaryotes by Using In-Cell NMR Spectroscopy (David S. Burz, Christopher M. DeMott, Asma Aldousary, Stephen Dansereau, Alexander Shekhtman)....Pages 423-444
Back Matter ....Pages 445-446

✦ Subjects


Imaging;Radiology


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