Metabolomics: Methods and Protocols

✍ Scribed by Sanjoy K. Bhattacharya

• Publisher: Springer New York; Humana
• Year: 2019
• Tongue: English
• Leaves: 336
• Series: Methods in Molecular Biology 1996
• Edition: 1st ed.
• Category: Library

✦ Synopsis

The volume brings together some of the best experts in the field of modern metabolomics to discuss various techniques used today to study specific metabolite classes, and metabolomics in bacterial systems and mammalian systems. The chapters in this book cover topics such as Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol and derivatives in ocular tissues using LC-MS/MS methods; microbial siderophores analysis by mass spectrometry; the metabolomic study of tissues in Parkinson’s Disease; and NMR analysis in livestock metabolomics. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, tips on troubleshooting and avoiding pitfalls.

Cutting-edge and thorough, Metabolomics: Methods and Protocols is an essential resource for any researcher interested in this exciting and evolving field.

✦ Table of Contents

Front Matter ....Pages i-xv
Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer (Lukáš Najdekr, Giovanny Rodriguez Blanco, Warwick B. Dunn)....Pages 1-15
Isotopic Ratio Outlier Analysis (IROA) for Quantitative Analysis (Chris Beecher, Felice A. de Jong)....Pages 17-28
Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals (Mukundan Ragavan, Matthew E. Merritt)....Pages 29-40
Quantitative Metabolomics Using Isotope Residue Outlier Analysis (IROA®) with Internal Standards (Roberto Mendez, Maria del Carmen Piqueras, Alexander Raskind, Felice A. de Jong, Chris Beecher, Sanjoy K. Bhattacharya et al.)....Pages 41-46
Analyses of Cholesterol Metabolites of Optic Nerve Using GC-MS Methods (Gurkaran S. Sarohia, Erik D. Garza, Sanjoy K. Bhattacharya)....Pages 47-51
Analyses of Cholesterol and Derivatives in Ocular Tissues Using LC-MS/MS Methods (Maria Piqueras, Despoina Theotoka, Gurkaran S. Sarohia, Sanjoy K. Bhattacharya)....Pages 53-59
Measurement of Pyridine Nucleotides in Biological Samples Using LC-MS/MS (Chris Petucci, Jeffrey A. Culver, Nidhi Kapoor, E. Hampton Sessions, Daniela Divlianska, Stephen J. Gardell)....Pages 61-73
Handling and Analysis of 5-Formyl-, 5,10-Methenyl-, 10-Formyl-, 5-Formimno-, and 5,10-Methylenetetrahydrofolates (Anita H. Lewin, Peter Silinski)....Pages 75-94
Protein–Lipid Complex Separation Utilizing a Capillary Electrophoresis System (Anddre Osmar Valdivia, Ciara Myer, Maria Fernanda Suarez, Sanjoy K. Bhattacharya)....Pages 95-100
Immature and Mature Collagen Crosslinks Quantification Using High-Performance Liquid Chromatography and High-Resolution Mass Spectrometry in Orbitrap™ (Diana R. Hernandez, Maria del Carmen Piqueras, Alejandro E. Macias, Laisel Martinez, Roberto Vazquez-Padron, Sanjoy K. Bhattacharya)....Pages 101-111
Quantitation of Neurotoxic Metabolites of the Kynurenine Pathway by Laser Desorption Ionization Mass Spectrometry (LDI-MS) (Pallavi Lahiri, Deepika Dhaware, Ajeet Singh, Venkateswarlu Panchagnula, Dipankar Ghosh)....Pages 113-129
Analysis of Microbial Siderophores by Mass Spectrometry (Tomas Pluhacek, Anton Skriba, Jiri Novak, Dominika Luptakova, Vladimir Havlicek)....Pages 131-153
HSD18B7 Enzyme Assay Technique Using a Triple Quadrupole Mass Spectrometer (Carina Wu, Nhuong-Sao Ton, Maria Fernanda Suarez, Maria Piqueras, Sanjoy K. Bhattacharya)....Pages 155-159
Assays for Intracellular Cyclic Adenosine Monophosphate (cAMP) and Lysosomal Acidification (Chiranjit Maity, Dipankar Ghosh, Sonia Guha)....Pages 161-178
Isotopic Ratio Outlier Analysis (IROA) of Aqueous Humor for Metabolites (Maria Del Carmen Piqueras, Ciara Myer, Anna Junk, Sanjoy K. Bhattacharya)....Pages 179-185
Assessment of Transport of Lipid Metabolites Within Trabecular Meshwork Cells (Daphne Toglia, Stephanie Dancausse, Karthik Kalahasty, Bryan Alfonso, Sanjoy K. Bhattacharya)....Pages 187-197
Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes (Shamroop Kumar Mallela, Devang Maheshkumar Patel, Gloria Michelle Ducasa, Sandra Merscher, Alessia Fornoni, Hassan Al-Ali)....Pages 199-206
Labeling Cancer Stem Cells with 13C6 Glucose and 13C5 Glutamine for Metabolic Flux Analysis (Kousik Kesh, Sulagna Banerjee)....Pages 207-216
Metabolomics Analyses from Tissues in Parkinson’s Disease (Fatema Bhinderwala, Shulei Lei, Jade Woods, Jordan Rose, Darrell D. Marshall, Eli Riekeberg et al.)....Pages 217-257
Application of Stable Isotope Labels for Metabolomics in Studies in Fatty Liver Disease (Patrycja Puchalska, Peter A. Crawford)....Pages 259-272
Proteometabolomics of Melphalan Resistance in Multiple Myeloma (David C. Koomen, Joy D. Guingab-Cagmat, Paula S. Oliveira, Bin Fang, Min Liu, Eric A. Welsh et al.)....Pages 273-296
Metabolomics Analyses of Muscle Atrophy Induced by Hind Limb Unloading (Stephen J. Gardell, Xiaolei Zhang, Nidhi Kapoor, Christopher Petucci, Paul M. Coen)....Pages 297-309
Protocols for NMR Analysis in Livestock Metabolomics (Aidin Foroutan, Seyed Ali Goldansaz, Matthias Lipfert, David S. Wishart)....Pages 311-324
Metabolomics Applicable to Retinal Vascular Diseases (Satish Patnaik, Subhadra Jalali, Manjunath B. Joshi, Kapaettu Satyamoorthy, Inderjeet Kaur)....Pages 325-331
Back Matter ....Pages 333-335

✦ Subjects

Life Sciences; Metabolomics; Mass Spectrometry