Clinical Metabolomics: Methods and Protocols

✍ Scribed by Martin Giera (eds.)

• Publisher: Humana Press
• Year: 2018
• Tongue: English
• Leaves: 387
• Series: Methods in Molecular Biology 1730
• Edition: 1
• Category: Library

✦ Synopsis

This detailed volume presents a comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations. The methodologies explored here form the core of several very promising initiatives evolving around personalized health care and precision medicine, which can be seen as complimentary to the field of clinical chemistry and aid the aforementioned field with novel disease markers and diagnostic patterns. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Clinical Metabolomics: Methods and Protocols aims to serve as the basis for successful communication between scientists from several fields, including chemists, biologist, bioinformaticians, and clinicians, ultimately leading to effective study design and completion.

✦ Table of Contents

Front Matter ....Pages i-xiv
Front Matter ....Pages 1-1
Metabolomics as a Tool to Understand Pathophysiological Processes (Julijana Ivanisevic, Aurelien Thomas)....Pages 3-28
Metabolomics in Immunology Research (Bart Everts)....Pages 29-42
Front Matter ....Pages 43-43
LC-MS-Based Metabolomics of Biofluids Using All-Ion Fragmentation (AIF) Acquisition (Romanas Chaleckis, Shama Naz, Isabel Meister, Craig E. Wheelock)....Pages 45-58
Lipid Mediator Metabolomics Via LC-MS/MS Profiling and Analysis (Jesmond Dalli, Romain A. Colas, Mary E. Walker, Charles N. Serhan)....Pages 59-72
UHPSFC/ESI-MS Analysis of Lipids (Miroslav Lísa, Michal Holčapek)....Pages 73-82
LC-MS/MS Analysis of Lipid Oxidation Products in Blood and Tissue Samples (Yiu Yiu Lee, Jetty Chung-Yung Lee)....Pages 83-92
Serum Testosterone by Liquid Chromatography Tandem Mass Spectrometry for Routine Clinical Diagnostics (Lennart J. van Winden, Olaf van Tellingen, Huub H. van Rossum)....Pages 93-102
LC-MS/MS Analysis of Bile Acids (Sabrina Krautbauer, Gerhard Liebisch)....Pages 103-110
LC-MS/MS Analysis of Triglycerides in Blood-Derived Samples (Madlen Reinicke, Susen Becker, Uta Ceglarek)....Pages 111-121
LC-MS/MS Analysis of the Epoxides and Diols Derived from the Endocannabinoid Arachidonoyl Ethanolamide (Amy A. Rand, Patrick O. Helmer, Bora Inceoglu, Bruce D. Hammock, Christophe Morisseau)....Pages 123-133
Sphingolipid Analysis in Clinical Research (Bo Burla, Sneha Muralidharan, Markus R. Wenk, Federico Torta)....Pages 135-162
Shotgun Lipidomics Approach for Clinical Samples (Lars F. Eggers, Dominik Schwudke)....Pages 163-174
Establishing and Performing Targeted Multi-residue Analysis for Lipid Mediators and Fatty Acids in Small Clinical Plasma Samples (Theresa L. Pedersen, John W. Newman)....Pages 175-212
Chemical Isotope Labeling LC-MS for Human Blood Metabolome Analysis (Wei Han, Liang Li)....Pages 213-225
Direct Infusion-Tandem Mass Spectrometry (DI-MS/MS) Analysis of Complex Lipids in Human Plasma and Serum Using the Lipidyzer™ Platform (Baljit K. Ubhi)....Pages 227-236
Front Matter ....Pages 237-237
Exploratory GC/MS-Based Metabolomics of Body Fluids (Carole Migné, Stéphanie Durand, Estelle Pujos-Guillot)....Pages 239-246
GC-MS Analysis of Short-Chain Fatty Acids in Feces, Cecum Content, and Blood Samples (Lisa R. Hoving, Marieke Heijink, Vanessa van Harmelen, Ko Willems van Dijk, Martin Giera)....Pages 247-256
GC-MS Analysis of Medium- and Long-Chain Fatty Acids in Blood Samples (Lisa R. Hoving, Marieke Heijink, Vanessa van Harmelen, Ko Willems van Dijk, Martin Giera)....Pages 257-265
Analysis of Oxysterols (Fabien Riols, Justine Bertrand-Michel)....Pages 267-275
Analysis of Metabolites from the Tricarboxylic Acid Cycle for Yeast and Bacteria Samples Using Gas Chromatography Mass Spectrometry (Reza Maleki Seifar, Angela ten Pierick, Patricia T. N. van Dam)....Pages 277-282
GC-MS Analysis of Lipid Oxidation Products in Blood, Urine, and Tissue Samples (Anne Barden, Trevor A. Mori)....Pages 283-292
Front Matter ....Pages 293-293
Metabolic Profiling of Urine by Capillary Electrophoresis-Mass Spectrometry Using Non-covalently Coated Capillaries (Rawi Ramautar)....Pages 295-304
CE-MS for the Analysis of Amino Acids (Karina Trevisan Rodrigues, Marina Franco Maggi Tavares, Ann Van Schepdael)....Pages 305-313
Front Matter ....Pages 315-315
NMR Analysis of Fecal Samples (Hye Kyong Kim, Sarantos Kostidis, Young Hae Choi)....Pages 317-328
Quantitative Analysis of Central Energy Metabolism in Cell Culture Samples (Sarantos Kostidis)....Pages 329-342
Front Matter ....Pages 343-343
Mass Spectrometry Imaging of Metabolites (Benjamin Balluff, Liam A. McDonnell)....Pages 345-357
Front Matter ....Pages 359-359
Quality-Assured Biobanking: The Leiden University Medical Center Model (Rianne Haumann, Hein W. Verspaget)....Pages 361-370
Extracting Knowledge from MS Clinical Metabolomic Data: Processing and Analysis Strategies (Julien Boccard, Serge Rudaz)....Pages 371-384
Back Matter ....Pages 385-389

✦ Subjects

Biochemistry