Nitration Reaction Catalyzed by Horseradish Peroxidase in the Presence of H2O2 and NaNO2

✍ Scribed by Rong-Ji DAI; Hui HUANG; Jie CHEN; Yu-Lin DENG; Sheng-Yuan XIAO

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 113 KB
• Volume: 25
• Category: Article
• ISSN: 0256-7660
• DOI: 10.1002/cjoc.200790312

✦ Synopsis

Abstract

The enzymatic nitration of phenol and m‐cresol catalyzed by horseradish peroxidase was studied in the presence of H~2~O~2~ and NaNO~2~. The results showed that the nitration products of phenol were 2‐nitro and 4‐nitrophenols. There was also a small amount of by‐products of hydroquinone and catechol. The influences of various reaction parameters, including pH, organic solvent type, and concentrations of NaNO~2~ and H~2~O~2~, on the nitration products were investigated. The yields of 4‐nitrophenol and 2‐nitrophenol were 14% and 12%, respectively. The nitration products of m‐cresol were 4‐nitro‐m‐cresol and 6‐nitro‐m‐cresol, and the yields of 4‐nitro‐m‐cresol and 6‐nitro‐m‐cresol were 19% and 30%, respectively.