Ninhydrin as a reagent for N-methyl-amino acids
β Scribed by D.W. Russell
- Publisher
- Elsevier Science
- Year
- 1960
- Tongue
- English
- Weight
- 210 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1873-3778
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β¦ Synopsis
SI-IORT COMMUNICATIONS
π SIMILAR VOLUMES
BmP (bromo tris(dimethylamino) phosphonium hexafluorophosphate) is a particularly suitable reagent for coupling N-methylated amino acids. It is stable and gives very high yields in short reaction times. Dipeptides are obtained without appreciable epimerization.
The reaction with indane-1,2,3-trione-2-hydrate (ninhydrin) is firmly established for the calorimetric determination of a-amino acids (l-3). The intensity of the blue-violet color produced is measured at about 570 rnp. This color is most intense when the reaction with ninhydrin is carried out at abo
A ninhydrin-orthophthalaldehyde reagent for the determination of NV-methylhistidine has been developed. The sensitivity of the reagent is similar to that of ninhydrin. Since most amino acids are not detected by the reagent, this allows the determination of Nr-methylhistidine in the presence of high