A ninhydrin-orthophthalaldehyde reagent for the determination of Nτ-methylhistidine
✍ Scribed by Leigh C. Ward
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 349 KB
- Volume
- 88
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A ninhydrin-orthophthalaldehyde reagent for the determination of NV-methylhistidine has been developed. The sensitivity of the reagent is similar to that of ninhydrin. Since most amino acids are not detected by the reagent, this allows the determination of Nr-methylhistidine in the presence of high concentrations of other amino acids. The reagent has been successfully used for the analysis of iV'-methylhistidine in human urine.
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