A method is presented for screening immobilized dyes applicable to the purification of enzymes from haemolysate (haemolysate can be considered as a nearly pure solution of haemoglobin containing only marginal amounts of enzymes). Haemolysate is loaded on immobilized dye mini-columns until haemoglobi
New method for the purification of enzymes
โ Scribed by J.S.H.
- Publisher
- Elsevier Science
- Year
- 1933
- Tongue
- English
- Weight
- 48 KB
- Volume
- 216
- Category
- Article
- ISSN
- 0016-0032
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โฆ Synopsis
has devised a new procedure for the purification of enzymes, the use of specific antienzymes as precipitants. Thus the urease of the soy bean was precipitated from aqueous extracts of that bean by the addition of highly purified antiurease. Washing the precipitate with 0.4 percent. sodium chloride solution removes the antiurease. The number of urease units per gram of ash-free solids is increased 850 times by this procedure.
J. S. H.
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