A new method for purification of mature elastin

✍ Scribed by B.L. Rasmussen; E. Bruenger; L.B. Sandberg

• Publisher: Elsevier Science
• Year: 1975
• Tongue: English
• Weight: 211 KB
• Volume: 64
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(75)90426-1

✦ Synopsis

The isolation of elastin from various tissues has depended upon its chemical inertness and resistance to hydrolysis. However, the majority of the procedures used to obtain a purified product are drastic enough that breakdown of elastin occurs. This communication describes a new method for the isolation of elastin with less degradation than existing methods. The tissue is milled through a 40 mesh screen in a Wiley Mill. It is extracted with guanidinium chloride, autoclaved, and then treated with formic acid-cyanogen bromide. A purified product is obtained and quantitation of NH~-terminal residues shows five to ten times less end-groups than elastin extracted with currently existing methods.