New strategies for the screening of a large number of immobilized dyes for the purification of enzymes : Application to the purification of enzymes from human haemolysate

✍ Scribed by Y. Kroviarski; S. Cochet; C. Vadon; A. Truskolaski; P. Boivin; O. Bertrand

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 689 KB
• Volume: 449
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)94403-0

✦ Synopsis

A method is presented for screening immobilized dyes applicable to the purification of enzymes from haemolysate (haemolysate can be considered as a nearly pure solution of haemoglobin containing only marginal amounts of enzymes). Haemolysate is loaded on immobilized dye mini-columns until haemoglobin and the studied enzymes are found in the column eluate at the same concentrations as those present in the haemolysate. Such a frontal mode of screening allows those dyes to be selected which, displaying a higher affinity for the enzyme of interest than for haemoglobin, can be used to displace the unwanted protein (haemoglobin) from the column by the enzyme of interest (present at a much lower concentration).