New method for the determination of pancreatic amylase evaluated

✍ Scribed by Klaus Lorentz; Dieter Seiler; Dietmar Nagel; Ulrich Naegele; Peter Koller; Renze Bais; Peter M. Bayer; Herbert Keller; Matti Puukka; Juhani Ruostesuo; Raija Puukka; Sidney B. Rosalki; Ying Foo

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 322 KB
• Volume: 5
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860050609

✦ Synopsis

We have carried out a multicenter evaluation of a new reagent carrier for Reflotronm', specific for pancreatic amylase where the salivary isoenzyme is inhibited by two specific monoclonal antibodies. This new procedure combines easy handling with low imprecision (median CV < 3%) in control material, serum, heparinized blood, and plasma) and close correlation (r = 0.991 to 0.999) with established manual and automated methods. The same close correlation was found with values obtained from either venous or capillary finger-stick blood. Salivary amylase up to 54 kUiL (37°C) was inhibited to about 97%.

Endogenous interference by hemoglobin, bilirubin, triglycerides, cholesterol, or hematocrit was found to be negligible within a wide range of interferent concentrations. Out of a panel of 28 commonly used drugs it was shown that only two (ascorbic acid and paracetamol), and then only at toxic concentrations, caused a deviation in amylase activity of greater than 10%. From the results of this study we conclude that this new method is suitable for highly precise and accurate measurements of pancreatic amylase in emergency and routine laboratories.