## Preparation of Starch Gels Containing 8 Molar Urea Starch gels suitable for electrophoresis of proteins according to Smithies (1) can readily be prepared using buffer solutions containing up to 8 M urea (e.g., reference 2). The presence of urea in the starch gels has been shown to improve resol
New fluorometric method for estimation of citrovorum factor
โ Scribed by M.S. Netrawali; R. Radhakrishnamurty; A. Sreenivasan
- Publisher
- Elsevier Science
- Year
- 1964
- Tongue
- English
- Weight
- 469 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A new fluorometric assay method for quinolinic acid is introduced in this study. Quinolinic acid-hydrazine complex, a stable fluorescent compound, is formed after heating quinolinic acid with hydrazine at 215-220ยฐC for 2 min. Fluorescence excitation and emission maxima of the complex are at 285 and
The fluorometric estimation has been carried out in a Technicon autoanalyzer system with a Sampler II (Technicon Inc.), an ISMATEC XIP 13 G.JlO Pump (Ismatec SA, Ziirich), an oil bath (Technicon Inc.
An interesting observation reported in our previous studies (1,2) is that, on binding to nucleic acids (NA) the dye, ethidium bromide (EB) increases its fluorescence quantum efficiency by a factor of 20 to 25. In consequence of the EB absorption spectrum shift on binding, for a suitably chosen wavel