Improved method for the fluorometric estimation of spermidine

✍ Scribed by Brett Elliott; I.Arthur Michaelson

• Publisher: Elsevier Science
• Year: 1967
• Tongue: English
• Weight: 198 KB
• Volume: 19
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(67)90149-2

✦ Synopsis

Preparation of Starch Gels Containing 8 Molar Urea

Starch gels suitable for electrophoresis of proteins according to Smithies (1) can readily be prepared using buffer solutions containing up to 8 M urea (e.g., reference 2). The presence of urea in the starch gels has been shown to improve resolution of proteins in some cases (e.g., reference 3) and can also be used to eliminate multiple bands when these are due to different degrees of aggregation of a single subunit. However, difficulties arise if gels of accurately controlled pH and ionic