New computational evidence for the catalytic mechanism of carbonic anhydrase
โ Scribed by Gian Pietro Miscione; Marco Stenta; Domenico Spinelli; Ernst Anders; Andrea Bottoni
- Publisher
- Springer
- Year
- 2007
- Tongue
- English
- Weight
- 770 KB
- Volume
- 118
- Category
- Article
- ISSN
- 1432-2234
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A new model for catalysis of human carbonic anhydrase II is suggested. The model is based on the X-ray structure of the hydrogen bond network in the catalytic site. The outer part of the network is proposed to adjust the pK a of the catalytic site to the experimentally observed value of about 7. The
## Abstract Carbonic anhydrases (CAs, EC 4.2.1.1) are a family of enzymes widespread in all life kingdoms. In mammals, isozyme CA IX is highly overexpressed in many cancer types being present in few normal tissues. Its expression is strongly induced by hypoxia present in many tumors, being regulate