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Hydrogen Bonds and the Catalytic Mechanism of Human Carbonic Anhydrase II

โœ Scribed by SILKE THOMS

Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
142 KB
Volume
215
Category
Article
ISSN
0022-5193

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โœฆ Synopsis

A new model for catalysis of human carbonic anhydrase II is suggested. The model is based on the X-ray structure of the hydrogen bond network in the catalytic site. The outer part of the network is proposed to adjust the pK a of the catalytic site to the experimentally observed value of about 7. The inner part of the network is proposed to become a low-barrier hydrogen bond network in the transition state. The energy released in forming the lowbarrier hydrogen bond network is used to catalyse the interconversion of CO 2 and HCO ร€ 3 : The suggested molecular mechanism is consistent with the generally accepted kinetic scheme for human carbonic anhydrase II.

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