New catalytic mechanism for human purine nucleoside phosphorylase
✍ Scribed by Fernanda Canduri; Valmir Fadel; Luiz Augusto Basso; Mário Sérgio Palma; Diógenes Santiago Santos; Walter Filgueira de Azevedo Jr.
- Book ID
- 116289747
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 542 KB
- Volume
- 327
- Category
- Article
- ISSN
- 0006-291X
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We have developed a new assay for purine nucleoside phosphorylase which is based on the release of tritium when [2-3H]inosine is used as the substrate and the reaction is coupled with xanthine oxidase. After the reaction is terminated, residual [2-3H]inosine is adsorbed on charcoal and the supernata
## Abstract ## Background Purine nucleoside phosphorylase (PNP) deficiency causes the accumulation of toxic purine metabolites and lethal T cell immune defects, which might be corrected by expressing PNP by transplanting bone marrow (BM) cells transduced with lentiviral vectors containing the huma