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A new ultramicrochemical assay for purine nucleoside phosphorylase

✍ Scribed by M.P. Uitendaal; C.H.M.M. de Bruyn; T.L. Oei; P. Hösli; C. Griscelli

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
375 KB
Volume
84
Category
Article
ISSN
0003-2697

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✦ Synopsis

An ultramicrochemical method using radioactive substrates has been developed for the quantitation of purine nucleoside phosphorylase (NP) activity in single cells. NP activity can reproducibly be measured in both directions of the enzyme reaction. A quantitative relationship between the number of cells incubated and the NP activity was found. Fibroblasts from a patient with impaired T-lymphocyte function showed a substantial reduction of NP activity (both directions). In contrast to previously available methods, the present ultramicro approach makes it possible to analyze normal and immunodeficient single B and T lymphocytes.

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