Cellular ATP is commonly determined as production of bioluminescence using a luciferin-luciferase reaction system. Before the measurement of bioluminescence, cellular ATP must first be extracted. Two commonly used extraction methods are: () Tris-borate buffer (pH 9.2) coupled with a heating process
New assay for ATP-sulphurylase using the luciferin-luciferase method
β Scribed by G.J.E. Balharry; D.J.D. Nicholas
- Book ID
- 102983959
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 983 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0003-2697
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The reaction kinetics of crude firefly lantern extracts with and without added synthetic luciferin were examined. The addition of exogenous luciferin to the reaction mixture resulted in an apparent increase in net light emission per unit of ATP in solution. This additional reactivity (up to 1000-fol
## Interference by a Bacterial Phosphatase Enzyme Stable to Perchlorate Treatment In an investigation of the nucieotide pools in Bacillus brevis we have observed the presence of an unusual ATP-degrading enzyme. This enzyme appears to be stable to perchlorate precipitation and neutralisation proced