✦ LIBER ✦

Effects of luciferin concentration of the quantitative assay of ATP using crude luciferase preparations

✍ Scribed by D.M. Karl; O. Holm-Hansen

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 743 KB
Volume: 75
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90060-9

No coin nor oath required. For personal study only.

✦ Synopsis

The reaction kinetics of crude firefly lantern extracts with and without added synthetic luciferin were examined. The addition of exogenous luciferin to the reaction mixture resulted in an apparent increase in net light emission per unit of ATP in solution. This additional reactivity (up to 1000-fold) enables the detection of subpicogram levels of ATP. The effects of enzyme preparation, dilution, and aging procedures on the increased sensitivity of the ATP assay, as well as the assay limitations of the crude firefly lantern extracts, are also discussed.

📜 SIMILAR VOLUMES

A Convenient One-Step Extraction of Cell

A Convenient One-Step Extraction of Cellular ATP Using Boiling Water for the Luciferin–Luciferase Assay of ATP

✍ Nae-Cherng Yang; Wai-Meng Ho; Yu-Hsuan Chen; Miao-Lin Hu 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 58 KB

Cellular ATP is commonly determined as production of bioluminescence using a luciferin-luciferase reaction system. Before the measurement of bioluminescence, cellular ATP must first be extracted. Two commonly used extraction methods are: () Tris-borate buffer (pH 9.2) coupled with a heating process

Use of the luciferin-luciferase assay of

Use of the luciferin-luciferase assay of ATP for measuring the bacterial growth: Application to Escherichia coli

✍ A. Kahru; R. Vilu 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 340 KB 👁 2 views

Determination of picogram amounts of ATP

Determination of picogram amounts of ATP using the luciferin-luciferase enzyme system

✍ George E. Lyman; John P. DeVincenzo 📂 Article 📅 1967 🏛 Elsevier Science 🌐 English ⚖ 492 KB

Flow-injection bioluminescent determinat

Flow-injection bioluminescent determination of ATP based on the use of the luciferin-luciferase system

✍ Grith Gamborg; Elo H. Hansen 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 762 KB

A flow-injection system is described for assay of low levels of ATE as based on reaction with luciferase-luciferin with ensuing detection of the bioluminescence generated. The system employs a dual-injection valve which permits simultaneous injection of individually metered zones of the ATE-containi

Advance in water quality monitoring and

Advance in water quality monitoring and the use of firefly luciferine/luciferase/ ATP bioassay

✍ Nuri Malley; D.L. Henry 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 431 KB

Recent advances rn rapid clinical bacterial quantitation in urine have encouraged the development of automated in live-time bacterial level monitoring tn drinking water and in recycled/reused (sanitary waste) water. We have evaluated the methodology and ins:rumentation used in the bacterial ATP dete

Simple assay of 0.1–1.0 pmol of ATP, ADP

Simple assay of 0.1–1.0 pmol of ATP, ADP, and AMP in single somatic cells using purified luciferin luciferase

✍ Horst Spielmann; Ursula Jacob-Müller; Petra Schulz 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 555 KB

The sensitivity of ATP determinations with crude firefly luciferin luciferase is limited by contaminating ATP converting enzymes, which cause a rapid decrease of the ATP level during the assay. Purified luciferase has the advantage of producing an almost constant light intensity proportional to the