Advance in water quality monitoring and the use of firefly luciferine/luciferase/ ATP bioassay
✍ Scribed by Nuri Malley; D.L. Henry
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 431 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0011-9164
No coin nor oath required. For personal study only.
✦ Synopsis
Recent advances rn rapid clinical bacterial quantitation in urine have encouraged the development of automated in live-time bacterial level monitoring tn drinking water and in recycled/reused (sanitary waste) water. We have evaluated the methodology and ins:rumentation used in the bacterial ATP determination. Under appropriate assay conditions there is a linear correlation between ATP content and live bacteria. We are statisfied that the common indicatw, whenever live bacteria is being quantitated. is the responsible respiratory metabolite Adenosine Triphosphate (ATP) as measured in pg/ml per cell (e g.. fecal t: co/i= 0.5 x lOas ,og/cell) that was counted as bacterial poof indicator labeled as biomass.
When this information was coupled to the oxygen uptake rate (automated ionic sensor rapid detector in 60 mmutes) the biological oxygen demand (SOD) could be calculated as:
(CONSTANT) (OXYGEN UPTAKE RATE) SOD = (Biomass)