A rapid method for determining ATP by the firefly luciferin-luciferase system
β Scribed by H. A. Neufeld; R. D. Towner; Judith Pace
- Book ID
- 112652850
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 199 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1420-682X
No coin nor oath required. For personal study only.
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## Interference by a Bacterial Phosphatase Enzyme Stable to Perchlorate Treatment In an investigation of the nucieotide pools in Bacillus brevis we have observed the presence of an unusual ATP-degrading enzyme. This enzyme appears to be stable to perchlorate precipitation and neutralisation proced
A stopped-flow spectrometer is used for ATP assay by firefly luciferaseluciferin method. It allows one to record initial rise of the light intensity and to differentiate the light produced due to the conversion of ADP to ATP by nucleoside diphosphokinase in the firefly lantern when other nucleoside
EDTA, pH 7.4 (Na,H,EDTA adjusted with NaOH or Na,EDTA adjusted with. HCl). EDTA is not completely soluble in this mixture, and the precipitated solid becomes finely dispersed in fresh mixtures. Large clumps form after 30 min of storage and E-E was therefore used fresh. On mixing E-E with sample (or