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A rapid method for determining ATP by the firefly luciferin-luciferase system

✍ Scribed by H. A. Neufeld; R. D. Towner; Judith Pace


Book ID
112652850
Publisher
Springer
Year
1975
Tongue
English
Weight
199 KB
Volume
31
Category
Article
ISSN
1420-682X

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πŸ“œ SIMILAR VOLUMES


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## Interference by a Bacterial Phosphatase Enzyme Stable to Perchlorate Treatment In an investigation of the nucieotide pools in Bacillus brevis we have observed the presence of an unusual ATP-degrading enzyme. This enzyme appears to be stable to perchlorate precipitation and neutralisation proced

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A stopped-flow spectrometer is used for ATP assay by firefly luciferaseluciferin method. It allows one to record initial rise of the light intensity and to differentiate the light produced due to the conversion of ADP to ATP by nucleoside diphosphokinase in the firefly lantern when other nucleoside

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EDTA, pH 7.4 (Na,H,EDTA adjusted with NaOH or Na,EDTA adjusted with. HCl). EDTA is not completely soluble in this mixture, and the precipitated solid becomes finely dispersed in fresh mixtures. Large clumps form after 30 min of storage and E-E was therefore used fresh. On mixing E-E with sample (or