✦ LIBER ✦

Neutral lipid enzyme electrode based on ion-sensitive field effect transistors

✍ Scribed by Mamiko Nakako; Yoshio Hanazato; Mitsuo Maeda; Satoru Shiono

Publisher: Elsevier Science
Year: 1986
Tongue: English
Weight: 466 KB
Volume: 185
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(86)80044-7

No coin nor oath required. For personal study only.

✦ Synopsis

An enzyme electrode for neutral lipid determination based on hydrogen ion-sensitive field effect transistors (pH-FET's) IS described. The electrode is composed of two pH-FET's with an immobilized lipase membrane on one pH-FET, and a platinum wire. Triglycerides are solubilized with 10% (v/v) Triton X-100. The electrode is used to determine triglycerides over wide concentration ranges with response times of ca. 2 min. Relations between signal and the logarithm of the concentration are linear over the ranges lOO-400 mM triacetin, 3-50 mM tributylin and 0.6-3 mM triolein. In the case of triolein, the detection limit is 9 fig ml-' (signal/noise = 3:l). The effect of Triton X-100 on the electrode response is discussed.

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