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A microsensor for urea based on an ion-selective field effect transistor

✍ Scribed by Isao Karbue; Eiichi Tamiya; Jonathan M. Dicks; Masao Gotoh; Research

Publisher: Elsevier Science
Year: 1986
Tongue: English
Weight: 351 KB
Volume: 185
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(86)80046-0

No coin nor oath required. For personal study only.

✦ Synopsis

The pH response of a pH-sensitive fieldeffect transistor (FET) is not affected by a ca. l-pm thick membrane formed from r-aminopropyltriethoxysilane and glutaraldehyde over the gate insulator by a vapor deposition method. The response between pH 5.5 and 8.5 is ca. 61 mV pH-' at 37°C in 5 mM Tris-HCl buffer. When urease is immobilized on the membrane, the sensor gives a linear relationship between the initial rate of the output gate voltage change and the logarithmic value of urea concentration between 16.7 and 167 mM. Determination of urea is possible within 30 s. The optimum pH is 6.0-6.5, at 37°C. The system can be used for 20 days with only slight loss of enzymatic activity.

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