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Nano-electrospray tandem mass spectrometry for the analysis of neurosteroid sulphates†

✍ Scribed by William J. Griffiths; Suya Liu; Yang Yang; Robert H. Purdy; Jan Sjövall

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
546 KB
Volume
13
Category
Article
ISSN
0951-4198

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✦ Synopsis

Neurosteroids are synthesised in the central and peripheral nervous system or are derived from peripheral sources, and act in the nervous system. In the present study we have evaluated the potential for using nanoelectrospray (nano-ES) tandem mass spectrometry (MS/MS) for the structural analysis and detection of neurosteroids, in particular, steroid sulphates found in brain. Complete structural information can be obtained from 1 ng (3 pmol) of steroid sulphate, while fragment ions characteristic of the sulphate ester group can be obtained from only 3 pg (10 fmol) of sample. These values correspond to the expected quantities of steroid sulphates (e.g. pregnenolone sulphate) in about 100 mg and 300 mg of brain, respectively. Deuterated neurosteroid sulphates added to homogenised rat brain have been successfully analysed by nano-ES-MS/MS at a level of 50 pg/mg of brain.

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