Equilenin-2′-deoxynucleoside adducts: analysis with nano-liquid chromatography coupled to nano-electrospray tandem mass spectrometry

✍ Scribed by Jan Embrechts; Filip Lemière; Walter Van Dongen; Eddy L. Esmans

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 284 KB
• Volume: 36
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/jms.136

✦ Synopsis

Abstract

The interaction of 4‐hydroxy metabolites of estrogens with DNA leads to the formation of DNA adducts. These adducts are believed to play an important role in the incidence of breast and endometrial cancer. In order to be able to analyze these adducts in in vivo samples a method based upon the coupling of miniaturized liquid chromatography (LC) to electrospray tandem mass spectrometry (ES‐MS/MS) was developed for the analysis of the adducts formed with 4‐hydroxyequilenin. In vitro synthesized adducts obtained by the reaction of 4‐hydroxyequilenin with the main 2′‐deoxynucleosides were separated on a Hypersyl C~18~ BDS nano‐HPLC column (15 cm × 75 µm i.d.) at a flow‐rate of 300 nl min^−1^ using gradient elution with CH~3~OH–0.2% CH~3~COOH in H~2~O. The column was coupled, in combination with a column switching system, to a nano‐electrospray interface. Analysis of the low‐ and high‐resolution low‐energy collision‐activated dissociation product ion spectra of normal and deuterated adducts supported earlier data demonstrating equilenin to form different isomeric adducts, except with thymidine, for which no adducts were found. The nano‐HPLC column‐switching ES‐MS system was tested for its sensitivity on a triple‐quadrupole instrument, and detection limits down to 197 fg in the single reaction monitoring mode were obtained for semi‐preparatively isolated equilenin–2′‐deoxyguanosine adduct. Copyright © 2001 John Wiley & Sons, Ltd.