## Abstract | I. | Introduction | 332 | | II. | Techniques and Methods | 334 | | | A. Sample Preparation | 334 | | | B. ESI‐MS/MS | 336 | | | 1. Glycerophosphocholine (GPCho) Lipids | 336 | | | 2. Sphingomyelin | 341 | | | 3. Glycerophosphoethanolamine (GPEtn) Lipids | 342 |
Analysis of phospholipids using electrospray ionisation tandem mass spectrometry
✍ Scribed by Elif Buyukpamukcu; Jöerg Hau; Laurent-Bernard Fay; Fabiola Dionisi
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2007
- Tongue
- English
- Weight
- 800 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0956-666X
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Phospholipids have widespread use as surfactants and emulsifying agents, and as ingredients with nutritional benefits. We describe an approach for their analysis, based on separation by reversed‐phase HPLC and identification and quantification by electrospray ionization MS and MS/MS. It enables the identification of phospholipids by assessing their polar head and fatty acid moieties, as well as their quantitative analysis.
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