Differentiation of diiodothyronines using electrospray ionization tandem mass spectrometry

✍ Scribed by Yuntao Zhang; Abigail H. Conrad; Rudy Thoma; Gary W. Conrad

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 136 KB
• Volume: 41
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/jms.971

✦ Synopsis

Abstract

Diiodothyronines 3,5‐diiodothyronine (3,5‐T2), 3′,5′‐diiodothyronine (3′,5′‐T2), and 3,3′‐diiodothyronine (3,3′‐T2) are important metabolites of 3,5,3′‐triiodothyronine (T3) and 3,3′,5′‐triiodothyronine (rT3; reverse T3). In this paper, a novel and rapid method for identifying and quantifying 3,5‐T2, 3′,5′‐T2 and 3,3′‐T2 has been introduced using electrospray ionization tandem mass spectrometry (ESI‐MS/MS). Fragmentation patterns were proposed on the basis of our data obtained by ESI‐MS/MS. MS^2^ spectra in either negative ionization mode or positive ionization mode can be used to differentiate 3,5‐T2, 3′,5′‐T2 and 3,3′‐T2. On the basis of the relative abundance of fragment ions in MS^2^ spectra under the positive ionization mode, quantification of the 3,5‐T2, 3′,5′‐T2 and 3,3′‐T2 isomers in mixtures is also achieved without prior separation. Copyright © 2005 John Wiley & Sons, Ltd.