N -Trifluoroacetylamino Alcohols as Phosphodiester Protecting Groups in the Synthesis of Oligodeoxyribonucleotides
β Scribed by Wilk, Andrzej; Srinivasachar, Kasturi; Beaucage, Serge L.
- Book ID
- 120089443
- Publisher
- American Chemical Society
- Year
- 1997
- Tongue
- English
- Weight
- 114 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0022-3263
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π SIMILAR VOLUMES
Any oligodeoxyribonucleotide triester block with a 3' aryl phosphate end can be protected by condensing its phosphodiester N4,~3',~5'-tribensoylcytidine. terminal with the 2'-hydroxyl group of As the final step in deprotection of the modified block, mild treatment with Pb\* ion cleaves the new 3'-2
Susnnary: The putative intermediate bis(l,2,4-triazolide) derivative (I) is used in the stepwise synthesis in solution of the phosphorodithioate analogue (l0) of thymiaylyl-(3'+5')-thymidylyl-(3'+5')-thymidine; the 2,4-dinitrobensyl (Dnb) group is used to protect phosphorodithioate internucleotide l