N-terminal peptide fragments of lipocortin-1 inhibit a549 cell growth and block EGF-induced stimulation of proliferation

✍ Scribed by J. D. Croxtall; S. Waheed; Q. Choudhury; R. Anand; R. J. Flower

• Book ID: 102867903
• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: French
• Weight: 669 KB
• Volume: 54
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910540124

✦ Synopsis

Lipocortin

-I mediates growth inhibition of glucocorticoids in A549 cells by suppressing the release of PGE2 necessary for their proliferation. We now show that 2 peptide fragments derived from the N-terminal portion of lipocortin-I corresponding to amino-acids 13-25 and 21-33 also inhibited A549 cell growth and suppressed release of PGE2, whereas peptides 1-12 and 13-25 (Phe*'; in which the tyrosine at position 21 was replaced by a phenylalanine residue) were inactive. Similarly, peptide 21-33 (Phe2I) and a scrambled sequence of 13-25 failed to inhibit cell growth. Moreover, the EGF-induced stimulation of cell proliferation and PGE2 release in these cells was blocked by peptides 13-25 and 2 1-33, and also by peptides I-12, 13-25 (Phe2') and 21-33 (Phe2'), but not by a scrambled sequence of peptide 13-25.