N-ras gene activation in the rd human rhabdomyosarcoma cell line

✍ Scribed by Pierre Chardin; Patrick Yeramian; Pascal Madaule; Armand Tavitian

• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: French
• Weight: 604 KB
• Volume: 35
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910350513

✦ Synopsis

Abstract

A transforming N‐ras allele was molecularly cloned from the RD human rhabdomyosarcoma cell line, and the nature of its activation studied. Construction of chimeric recombinants between the RD‐transforming allele and a normal human allele enabled us to localize the alteration responsible for the activation to the second exon of the N‐ras gene. The nucleotide sequence of this exon, when compared to that of the normal allele, revealed a single difference at the 61st amino acid position of the encoded protein; the CAA codon for glutamine in the normal allele was mutated to a CAT codon for histidine in the RD‐transforming allele. This result is the first description of a histidine replacing glutamine in the 61st position and provides further evidence that the 61st amino acid is one of the preferential sites for N‐ras activation.