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Aphidicolin induces myogenic differentiation in the human rhabdomyosarcoma cell line KFR.

✍ Scribed by Jindrich Cinatl jr.; Jaroslav Cinatl; Pablo Hernáiz Driever; Holger Rabenau; Milos Novak; Milan Stefanik; Bernhard Kornhuber; Hans-Wilhelm Doerr

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
998 KB
Volume
18
Category
Article
ISSN
1065-6995

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✦ Synopsis

ABSTRACT

The effects of aphidicolin, a specific inhibitor of DNA polymerase α, on cell growth, DNA synthesis and myogenic differentiation in the human alveolar rhabdomyosarcoma cell line KFR were studied. The treatment with aphidicolin at 5 × 10^−6^ M concentration, which completely inhibited DNA synthesis and cell growth, induced morphological differentiation of small mononuclear cells to elongated, multinucleated (myotube‐like) structures. The morphological differentiation was accompanied by the expression of skeletal muscle myosin; about 30% myosin‐positive cells were observed after 14 days of treatment, compared to 2.3% in untreated cultures. The results showed that aphidicolin induces differentiation of human rhabdomyosarcoma cells and that multinucleated myotube‐like elements may develop simply by cell fusion without cell division and DNA synthesis.

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