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Overexpression of p21WAF1/CIP1 induces cell differentiation and growth inhibition in a human glioma cell line

✍ Scribed by Takashi Kokunai; Ichiro Izawa; Norihiko Tamaki

Publisher: John Wiley and Sons
Year: 1998
Tongue: French
Weight: 215 KB
Volume: 75
Category: Article
ISSN: 0020-7136
DOI: 10.1002/(sici)1097-0215(19980209)75:4<643::aid-ijc24>3.0.co;2-8

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✦ Synopsis

p21 WAF1/CIP1 is a downstream mediator of p53 and mediates growth arrest by inhibiting the action of G 1 cyclin-dependent kinases. Since cellular differentiation is frequently characterized by G 1 arrest, we examined whether p21 WAF1/CIP1 overexpression would induce growth suppression and differentiation in p53-defective human glioma cells. Overexpression of p21 WAF1/CIP1 resulted in an accumulation of cells in G 1 , altered morphology, growth arrest and cell differentiation. The extent of cell differentiation correlated with the level of p21 WAF1/CIP1 as well as of proliferating cell nuclear antigen, cyclin E, and cdk 2, which associates with p21 WAF1/CIP1 . Our data suggest that gene transfer of p21 WAF1/CIP1 may arrest glioma cell growth in vivo by committing malignant glioma cells to a pathway of terminal differentiation.

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