𝔖 Bobbio Scriptorium
✦   LIBER   ✦

N-Hydroxysuccinimidyl Fluorescein-O-acetate as a Fluorescent Derivatizing Reagent for Catecholamines in Liquid Chromatography

✍ Scribed by Hong Wang; Jin Li; Xin Liu; Ting-Xian Yang; Hua-Shan Zhang

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
85 KB
Volume
281
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A new amine-reactive derivatizing reagent, N-hydroxysuccinimidyl fluorescein-O-acetate (SIFA), was developed for catecholamine (CA) analysis in liquid chromatography. The reactivity of this reagent with the CAs norepinephrine (NE), epinephrine (E), and dopamine (DA) was investigated in detail. In aqueous methanol containing 32 mmol/L pH 9.0 H 3 BO 3 -Na 2 B 4 O 7 buffer, SIFA reacted with NE, E, and DA under mild conditions. The derivatives were separated in 20 min on a C 18 column with a mobile phase of methanol/water (38:62, v/v) containing 10 mmol/L pH 5.0 H 3 cit-Na 2 HPO 4 buffer. At ex / em ‫؍‬ 490/516 nm, the detection limits were 3.2, 12, and 56 fmol, respectively, with a signal-to-noise ratio of 3, which were comparable to those using 1,2-diphenylethylenediamine as the derivatizing reagent for CA analysis. Amino acids, aliphatic amines, and alcohols had no obvious interference with the determination. The proposed method has been applied to the determination of CAs in human urine, with recoveries of 95.3-103.9%.

📜 SIMILAR VOLUMES

Synthesis of a Fluorescent Derivatizing
Synthesis of a Fluorescent Derivatizing Reagent, 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate, and Its Application for the Analysis of Hydrolysate Amino Acids via High-Performance Liquid Chromatography
✍ S.A. Cohen; D.P. Michaud 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 700 KB

A highly reactive amine derivatizing reagent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate, has been synthesized. In a rapid, one-step procedure, the compound reacts with amino acids to form stable unsymmetric urea derivatives which are readily amenable to analysis by reversed phase HPLC. Studies

2,2′-Dithiobis(1-amino-4,5-dimethoxybenz
2,2′-Dithiobis(1-amino-4,5-dimethoxybenzene) as a highly sensitive, selective and stable fluorescence derivatization reagent for aromatic aldehydes in liquid chromatography
✍ Shuuji Hara; Masaru Nakamura; Fumie Sakai; Hitoshi Nohta; Yosuke Ohkura; Masatos 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 542 KB

2,2'-Dithiobiscl-amine-4,5-dimethoxybenzene) was synthesized as a highly sensitive, selective and stable fluorescence derivatization reagent for aromatic aldehydes in liquid chromatography. The reagent reacts selectively with aromatic aldehydes in acidic media in the presence of tri-n-butylphosphine

Synthesis of 4-[2-(N,N-dimethylamino)eth
Synthesis of 4-[2-(N,N-dimethylamino)ethylaminosulfonyl]-7-N-methylhydrazino-2,1,3-benzoxadiazole (DAABD-MHz) as a derivatization reagent for aldehydes in liquid chromatography/electrospray ionization–tandem mass spectrometry
✍ Tomofumi Santa; Osama Y Al-Dirbashi; Tomoko Ichibangase; Mohamed S Rashed; Takes 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 159 KB 👁 1 views

## Abstract Benzofurazan derivatization reagent, 4‐[2‐(__N__,__N__‐dimethylamino)ethylaminosulfonyl]‐7‐__N__‐methylhydrazino‐2,1,3‐benzoxadiazole (DAABD‐MHz), for aldehydes in liquid chromatography/electrospray ionization–tandem mass spectrometry (LC/ESI‐MS/MS), was synthesized. DAABD‐MHz reacted w