Synthesis of 4-[2-(N,N-dimethylamino)ethylaminosulfonyl]-7-N-methylhydrazino-2,1,3-benzoxadiazole (DAABD-MHz) as a derivatization reagent for aldehydes in liquid chromatography/electrospray ionization–tandem mass spectrometry

✍ Scribed by Tomofumi Santa; Osama Y Al-Dirbashi; Tomoko Ichibangase; Mohamed S Rashed; Takeshi Fukushima; Kazuhiro Imai

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 159 KB
• Volume: 22
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.903

✦ Synopsis

Abstract

Benzofurazan derivatization reagent, 4‐[2‐(N,N‐dimethylamino)ethylaminosulfonyl]‐7‐N‐methylhydrazino‐2,1,3‐benzoxadiazole (DAABD‐MHz), for aldehydes in liquid chromatography/electrospray ionization–tandem mass spectrometry (LC/ESI‐MS/MS), was synthesized. DAABD‐MHz reacted with aliphatic aldehydes under mild conditions. The generated derivatives were separated on a reversed‐phase column and detected by ESI‐MS/MS with detection limits of 30–60 fmol on‐column. Upon collision‐induced dissociation, a single and intense fragment ion at m/z 151 was observed. These results suggested that DAABD‐MHz was suitable as a derivatization reagent in LC/ESI‐MS/MS. Copyright © 2007 John Wiley & Sons, Ltd.