2,2′-Dithiobis(1-amino-4,5-dimethoxybenzene) as a highly sensitive, selective and stable fluorescence derivatization reagent for aromatic aldehydes in liquid chromatography

✍ Scribed by Shuuji Hara; Masaru Nakamura; Fumie Sakai; Hitoshi Nohta; Yosuke Ohkura; Masatoshi Yamaguchi

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 542 KB
• Volume: 291
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)85142-5

✦ Synopsis

2,2'-Dithiobiscl-amine-4,5-dimethoxybenzene) was synthesized as a highly sensitive, selective and stable fluorescence derivatization reagent for aromatic aldehydes in liquid chromatography. The reagent reacts selectively with aromatic aldehydes in acidic media in the presence of tri-n-butylphosphine, sodium sulphite and disodium hydrogenphosphite. The reaction was complete within 60 min at 37°C. The fluorescent products from benzaldehyde and 4-hydroxybenzaldehyde are shown to be 2-phenyl-5,6-dimethoxybenzothiazole and 2_(4-hydroxyphenyl)-5,6-dimethoxybenzothiazole, respectively. The fluorescent derivatives of aromatic aldehydes can be separated by reversed-phase chromatography and their detection limits (signal-to-noise ratio = 3) are 8-20 fmol on-column.