✦ LIBER ✦

2-Amino-4,5-ethylenedioxyphenol as fluorescence derivatization reagent for aromatic aldehydes in liquid chromatography

✍ Scribed by Hitoshi Nohta; Fumie Sakai; Masaaki Kai; Yosuke Ohkura; Mikihiko Saito

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 435 KB
Volume: 287
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(93)e0600-c

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✦ Synopsis

2-Amino-4,5ethylenedioxyphenol was found to be useful as a precolumn fluorescence derivatization reagent for aromatic aldehydes in liquid chromatography. The reagent reacts selectively with the aldehydes in two steps: first, heating WMIT) for 45 min in methanol solution in the presence of dicyclohexylcarbodiimide; secondly, standing for more than 1 min at room temperature after acidification. The fluorescent product from 4-hydroxybenzaldehyde is shown to be 2_(4-hydroxyphenyl)_5,6-ethylenedioxybenzoxazole. The fluorescent derivatives of aromatic aldehydes can be separated by reversed-phase chromatography and their detection limits (S/N = 3) are 5-10 pmol on column.

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