✦ LIBER ✦

2-(5-Hydrazinocarbonyl-2-thienyl) -5, 6-methylenedioxybenzofuran and 2-(5-hydrazinocarbonyl-2-furyl)-5, 6-methylenedioxybenzofuran as novel fluorescence derivatisation reagents for carboxylic acids in liquid chromatography

✍ Scribed by Mikihiko Saito; Tamano Ushijima; Kazumi Sasamoto; Kenshi Yakata; Yosuke Ohkura; Keiyu Ueno

Publisher: Elsevier Science
Year: 1995
Tongue: English
Weight: 756 KB
Volume: 300
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(94)00422-i

No coin nor oath required. For personal study only.

✦ Synopsis

The new acid hydrazides, 2-( S-hydrazinocarbonyl-2-thienyl) -5,6-methylenedioxybenzofuran (TMBH) and 2-( S-hydrazinocarbonyl-Zfuryl) -5,6-methylenedioxybenzofuran (FMBH) , were synthesized as precolumn fluorescence derivatisation reagents for carboxylic acids in liquid chromatography. These compounds readily react with carboxylic acids in the presence of a coupling reagent under mild conditions in aqueous solution to give fluorescent derivatives. The detection limits of lauric acid were both 0.1 pmol per 10 ~1 injection volume at a signal-to-noise ratio of 3. The methods in which these compounds are used were applied to the assay of a standard mixture of prostaglandins (25 PM) and prostaglandins in a human seminal fluid.

📜 SIMILAR VOLUMES

2,2′-Dithiobis(1-amino-4,5-dimethoxybenz

2,2′-Dithiobis(1-amino-4,5-dimethoxybenzene) as a highly sensitive, selective and stable fluorescence derivatization reagent for aromatic aldehydes in liquid chromatography

✍ Shuuji Hara; Masaru Nakamura; Fumie Sakai; Hitoshi Nohta; Yosuke Ohkura; Masatos 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 542 KB

2,2'-Dithiobiscl-amine-4,5-dimethoxybenzene) was synthesized as a highly sensitive, selective and stable fluorescence derivatization reagent for aromatic aldehydes in liquid chromatography. The reagent reacts selectively with aromatic aldehydes in acidic media in the presence of tri-n-butylphosphine

4-(5,6-Dimethoxy-2-phthalimidinyl)-2-met

4-(5,6-Dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl Chloride as a Fluorescent Labeling Reagent for Determination of Amino Acids in High-Performance Liquid Chromatography and Its Application for Determination of Urinary Free Hydroxyproline

✍ Yasuto Tsuruta; Hirofumi Inoue 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 106 KB

A highly sensitive fluorescent labeling reagent, 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride (DMS-Cl), for determination of amino acids by HPLC has been developed. DMS-Cl reacted with amino acids in the basic medium to produce the corresponding fluorescent sulfonamides (excit