N-Glycan fucosylation of epidermal growth factor receptor modulates receptor activity and sensitivity to epidermal growth factor receptor tyrosine kinase inhibitor
โ Scribed by Kazuko Matsumoto; Hideyuki Yokote; Tokuzo Arao; Mari Maegawa; Kaoru Tanaka; Yoshihiko Fujita; Chikako Shimizu; Toshiaki Hanafusa; Yasuhiro Fujiwara; Kazuto Nishio
- Book ID
- 108584229
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 378 KB
- Volume
- 99
- Category
- Article
- ISSN
- 1347-9032
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Activation of cells is frequently followed by tyrosine phosphorylation of proteins. To quantify this process, we developed a ratiometric enzyme-linked immunosorbent assay (ELISA) using epidermal growth factor receptors (EGFR) as a model. Microtiter dishes were coated with anti-EGFR monoclonal antibo
## Abstract For Abstract see ChemInform Abstract in Full Text.
The association/dissociation binding kinetics of 1251-labeled mouse epidermal growth factor (EGF) to receptors on human fibroblast cells in monolayer culture have been measured at 4ยฐC as a function of extracellular pH from pH 5-9. At pH 8, steady-state total binding is maximal. As pH is lowered to 6