Mutation detection by chemical cleavage
โ Scribed by R.G.H. Cotton
- Book ID
- 114342386
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 60 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1050-3862
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๐ SIMILAR VOLUMES
We have developed a solid phase chemical cleavage method (SpCCM) for screening large DNA fragments for mutations. All reactions can be carried out in microtiterwells from the first amplification of the patient (or test) DNA through the search for mutations. The reaction time is significantly reduced
## Communicated by Mark H. Paalman Mutations in the tumor-suppressor p53 gene TP53 are frequent in most human cancers including breast cancer. A new solid phase chemical cleavage of mismatch method (CCM) allowed rapid and efficient screening and analysis of the TP53 gene in DNA samples extracted
We have developed a strategy for the complete detection of point mutations, small insertions and deletions by chemical cleavage based on the methodology of Cotton et al. (1988). The technique was extended by the development of a nonisotopic cleavage product detection system using silver staining aft