✦ LIBER ✦

Sensitive and fast mutation detection by solid phase chemical cleavage

✍ Scribed by Lise Lotte Hansen; Just Justesen; Torben A. Kruse

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 685 KB
Volume: 7
Category: Article
ISSN: 1059-7794
DOI: 10.1002/(sici)1098-1004(1996)7:3<256::aid-humu10>3.0.co;2-1

No coin nor oath required. For personal study only.

✦ Synopsis

We have developed a solid phase chemical cleavage method (SpCCM) for screening large DNA fragments for mutations. All reactions can be carried out in microtiterwells from the first amplification of the patient (or test) DNA through the search for mutations. The reaction time is significantly reduced compared to the conventional chemical cleavage method (CCM), and even by using a uniformly labelled probe, the exact position and nature of the mutation can be revealed. The SpCCM is suitable for automatization using a workstation to carry out the reactions and a fluorescent detection-based DNA sequencing system to analyze the cleaved fragments.

📜 SIMILAR VOLUMES

Mutation detection by solid phase primer

Mutation detection by solid phase primer extension

✍ John M. Shumaker; Andres Metspalu; C. Thomas Caskey 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 856 KB

A mutation analysis method based upon a wild-type DNA sequence is presented. Oligonucleotides were utilized for primer extension by T7 DNA polymerase to discriminate between wild-type and mutant sequences in two solid phase approaches. 1. Oligonucleotides were annealed to an immobilized template, ex

Novel TP53 gene mutations in tumors of R

Novel TP53 gene mutations in tumors of Russian patients with breast cancer detected using a new solid phase chemical cleavage of mismatch method and identified by sequencing

✍ A. Lambrinakos; M. Yakubovskaya; J.J. Babon; A.A. Neschastnova; Ya.V. Vishnevska 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 281 KB 👁 2 views

## Communicated by Mark H. Paalman Mutations in the tumor-suppressor p53 gene TP53 are frequent in most human cancers including breast cancer. A new solid phase chemical cleavage of mismatch method (CCM) allowed rapid and efficient screening and analysis of the TP53 gene in DNA samples extracted

Detection of human cytomegalovirus-speci

Detection of human cytomegalovirus-specific igg antibodies by a sensitive solid-phase radioimmunoassay and by a rapid-screening test

✍ Nina Kimmel; Maureen G. Friedman; Israel Sarov 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 English ⚖ 511 KB

## Abstract A radioimmunoassay (RIA) for the detection of human IgG antibodies to cytomegalovirus (CMV) which utilizes extracts of CMV‐infected cells as antigen is described. Sera of 66 healthy adults were titered by the RIA and by the complement fixation (CF) test. These same subjects were also us