MUSIC in Triple-Resonance Experiments: Amino Acid Type-Selective 1H–15N Correlations

✍ Scribed by Mario Schubert; Maika Smalla; Peter Schmieder; Hartmut Oschkinat

• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 133 KB
• Volume: 141
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.1999.1881

✦ Synopsis

Amino acid type-selective triple-resonance experiments can be of great help for the assignment of protein spectra, since they help to remove ambiguities in either manual or automated assignment procedures. Here, modified triple-resonance experiments that yield amino acid type-selective 1 H-15 N correlations are presented. They are based on novel coherence transfer schemes, the MUSIC pulse sequence elements, that replace the initial INEPT transfer and are selective for XH 2 or XH 3 (X can be 15 N or 13 C). The desired amino acid type is thereby selected based on the topology of the side chain. Experiments for Gly (G-HSQC); Ala (A-HSQC); Thr, Val, Ile, and Ala (TAVI-HSQC); Thr and Ala (TA-HSQC), as well as Asn and Gln (N-HSQC and QN-HSQC), are described. The new experiments are recorded as two-dimensional experiments and therefore need only small amounts of spectrometer time. The performance of the experiments is demonstrated with the application to two protein domains.