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Triple-Resonance Experiments for Correlation of H5 and Exchangeable Pyrimidine Base Hydrogens in 13C,15N-Labeled RNA

✍ Scribed by Jens Wöhnert; Ramadurai Ramachandran; Matthias Görlach; Larry R. Brown

Publisher: Elsevier Science
Year: 1999
Tongue: English
Weight: 66 KB
Volume: 139
Category: Article
ISSN: 1090-7807
DOI: 10.1006/jmre.1999.1797

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✦ Synopsis

Triple-resonance two-dimensional H5(C5C4N)H experiments are described that provide through-bond H5 to imino/amino connectivities in uridines and cytidines in (13)C, (15)N-labeled RNAs. The experiments employ selective INEPT steps for transferring magnetization from the H5 hydrogens through the intervening C5, C4, and N3/N4 nuclei to the imino/amino hydrogens. The improved sensitivity of these experiments for assignments in a large 43-nucleotide RNA is demonstrated.

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