MUSIC and Aromatic Residues: Amino Acid Type-Selective 1H–15N Correlations, III

✍ Scribed by Mario Schubert; Hartmut Oschkinat; Peter Schmieder

• Publisher: Elsevier Science
• Year: 2001
• Tongue: English
• Weight: 195 KB
• Volume: 153
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.2001.2447

✦ Synopsis

Amino acid type-selective experiments can help to remove ambiguities in automated assignment procedures for 15 N/ 13 C-labeled proteins. Here we present five triple-resonance experiments that yield amino acid type-selective 1 H-15 N correlations for aromatic amino acids. Four of the novel experiments are based on the MUSIC coherence transfer scheme that replaces the initial INEPT transfer and is selective for CH 2 . The MUSIC sequence is combined with selective excitation pulses to create experiments for Trp (W-HSQC) as well as Phe, Tyr, and His (FYH-HSQC). In addition, an experiment selective for Trp H ε1 -N ε1 is presented. The new experiments are recorded as two-dimensional experiments and their performance is demonstrated with the application to a protein domain of 115 amino acids.