𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Murine sarcoma virus: Titration patterns and interference by a murine leukemia virus

✍ Scribed by K. D. Somers; W. H. Kirsten

Book ID
102863362
Publisher
John Wiley and Sons
Year
1969
Tongue
French
Weight
566 KB
Volume
4
Category
Article
ISSN
0020-7136

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✦ Synopsis

A murine sarcoma virus (MSV-K) was assayed by conversion of 333 mouse cells into foci of morphologically altered cells. Murine erythroblastosis virus ( M E V ) , a member of the murine leukemia virus group, induced resistance in 3T3 cultures specific to super-infection with focus-forming MSV-K.

Interference by MEV was first detected at 14 days and reached maximal levels 21 days post infection. MSV-K preparations from transformed 3T3 cells contained competent virions, as judged by '' one-hit '' titration patterns. Since excess '' helper " MEV in MSV-K stocks could effectively convert titration patterns to '' one-hit '' kinetics, the ratio of MEV to MSV-K was determined by interference experiments. The ratio of MEV to MSV was approximately 1O:I. Thus it appears that a single particle is capable of producing a focus of altered cells in the absence of simultaneous infection with a separate particle of ME V.

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