Deoxyglucose transport by uninfected, murihe sarcoma virus-transformed, and murine leukemia virus-infected mouse cells

✍ Scribed by Peter G. W. Plagemann

• Publisher: John Wiley and Sons
• Year: 1973
• Tongue: English
• Weight: 974 KB
• Volume: 82
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040820312

✦ Synopsis

The initial rates of deoxy-D-glucose transport by cultures of growing and density-inhibited mouse embryo cells and lines of mouse cells transformed spontaneously or after infection by murine leukemia virus or murine sarcoma virus were investigated as a function of the deoxyglucose concentration. The apparent K,,, for deoxyglucose transport was about the same for all types of cells (1-2 mM). The V, , , of secondary cultures of mouse embryo cells decreased from 6 nmoles/l06 cellslminute for sparse cultures to less than 1 nmolell06 cellslminute for density-inhibited cultures. The V, , , was about the same whether estimated in monolayer culture or in suspensions of cells dispersed by treatment with trypsin. The V, , , for deoxyglucose transport by the established cells, whether transformed spontaneously or by virus infection, was 4 to 25 times higher than that for density-inhibited mouse embryo cells and was independent of the cell density of the cultures. Deoxyglucose transport was competitively inhibited by Cytochalasin B, Persantin, glucose and 3-0methyl-D-glucose and the apparent K, values of inhibition were similar for the mouse embryo cells and the various cell lines. Similarly, the sensitivity of the glucose transport systems to inactivation by p-chloromercuribenzoate was about the same for all types of cells. The results suggest that the glucose transport system of the normal mouse embryo cells and the cells of the various established lines is qualitatively the same, but that the number of functional transport sites differs for the various cell lines and decreases markedly in mouse embryo cells with an increase in cell density of the cultures.