Transformation of a mouse cell line by murine sarcoma virus (Moloney)

✍ Scribed by Hiroshi Yoshikura; Yasuko Hirokawa; Yoji Ikawa; Haruo Sugano

• Publisher: John Wiley and Sons
• Year: 1968
• Tongue: French
• Volume: 3
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910030607

✦ Synopsis

Murine sarcoma virus (Moloney) ( M S V ) transformed C3H2K cells originating ,ji.otn the kidney tissues of a newborn C3HIHe mouse. Titration of tlze virus showed a one-hit dose response in the presence of an excess of Friend leukemia virus (FL V ) and a two-hit dose response in the absence of FLV, indicating that M S V was defective and a dual infection by M S V and a niurine leukemia virus was required for M S V focus formation. Preinfection with FL V interfered with focus formation by M S V . The M S V foci appeared most abundantly when the cells in the S phase were infected with the virus. Remarkable similarities between muvine and avian sarcoma viruses were thus established. Focus center assay on either uninfected or FL V-infected C3H2K cell monolayer indicated that M S V f o c i did not arise by cellular division alone but required secondary infection, while RSV foci arose by cellular division alone.